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Department of Biological and Chemical Engineering

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Engelbrecht, N., Ambrose, H. W., Sieborg, M. U. & Kofoed, M. V. W. (2026). Heat integration aspects of exothermic biomethanation: A pilot reactor with shell-and-tube heat exchange capability. Energy Conversion and Management, 353, Article 121168. https://doi.org/10.1016/j.enconman.2026.121168

Palacios, P. A. & Kofoed, M. V. W. (2026). Demonstration of H₂/CO₂ acetogenesis by Acetobacterium wieringae in a trickle-bed reactor without organic substrates. Chemical Engineering Journal Advances, 25, Article 101029. https://doi.org/10.1016/j.ceja.2025.101029

Palacios Jaramillo, P. A. & Kofoed, M. V. W. (2026). Performance of mesophilic and thermophilic acetogenic strains for CO2 -to-acetate production. Biomass and Bioenergy, 207, Article 108599. https://doi.org/10.1016/j.biombioe.2025.108599

Romio, C., Kofoed, M. V. W. & Møller, H. B. (2026). Alkaline treatment with KOH for enhanced biogas recovery from agricultural feedstock and digested residues. Biomass and Bioenergy, 209, Article 108913. https://doi.org/10.1016/j.biombioe.2025.108913

Ahmmed, M. S., Otten, C. K., Kofoed, M. V. W. & Batstone, D. J. (2025). Impact of rheology on mass transfer and bubble diameter in wastewater treatment systems. Chemical Engineering Research and Design, 224, 523-532. https://doi.org/10.1016/j.cherd.2025.11.039

Ambrose, H. W., Engelbrecht, N., Alamia, A. & Kofoed, M. V. W. (2025). Biomethanation With Renewable Energy: Harnessing the Carbon-Water-Energy Nexus. Abstract from 33rd European Biomass Conference & Exhibition , Valencia, Spain.

Ambrose, H. W., Feilberg, A., Schwab, C. & Kofoed, M. V. W. (2025). Impact of chemical and biological additives on microbial community and methane emissions in pig slurry storage. 41. Abstract from 13th International Symposium on Anaerobic Microbiology, Leipzig, Germany.

Ambrose, H. W., Bambace, M. F., Marietou, A., Hosek, J., Feilberg, A., Kofoed, M. V. W. & Schwab, C. (2025). Novel Biological Approach to Mitigate Methane Emissions from Livestock Slurry through Microbial Conversion of Glycerol. Environmental Science & Technology, 59(38), 20512-20525. https://doi.org/10.1021/acs.est.4c12999

Calegari, R. P., Schutgens, M., Ottosen, L. D. M. & Kofoed, M. V. W. (2025). Selenium and Cysteine Synergy: Enhancing methane production rates in hyperthermophilic methanogens. 1604. Abstract from FEMS MICRO 2025, Milan, Italy.

Jensen, T. B., Anand, M., Batstone, D. & Kofoed, M. V. W. (2025). Here comes the sun: intermittent operation of hydrogen-based in situ biomethanation at pilot-scale. Applied Energy, 401(Part A), Article 126601. https://doi.org/10.1016/j.apenergy.2025.126601

Nielsen, A. K. H., Ottosen, L. D. M. & Kofoed, M. V. W. (2025). Production of acetate from flue gas-CO₂ by membrane-based bio-integrated carbon capture and utilization. Chemical Engineering Journal, 526, Article 170633. https://doi.org/10.1016/j.cej.2025.170633

Palacios Jaramillo, P. A., Sieborg, M. U., Kuipers, S. B., Fruergaard, S. & Kofoed, M. V. W. (2025). Temperature tactics: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures. Biochemical Engineering Journal, 214, Article 109574. https://doi.org/10.1016/j.bej.2024.109574

Sieborg, M. U. & Kofoed, M. V. W. (2025). Gamle processer, nye muligheder: Nyt kemisk-biologisk koncept til CO2-fangst og omdannelse. Dansk Kemi, 106(2), 12-14. Article 106. https://ipaper.ipapercms.dk/TechMedia/DanskKemi/2025/2/?page=12

Sieborg, M. U., Engelbrecht, N., Singh, A., Schnürer, A., Ottosen, L. D. M. & Kofoed, M. V. W. (2025). Unraveling the effects of temperature on mass transfer and microbiology in thermophilic and extreme thermophilic trickle bed biomethanation reactors. Chemical Engineering Journal, 509, Article 161179. https://doi.org/10.1016/j.cej.2025.161179

Wijesooriya, M. M., Calegari, R. P., Kofoed, M. V. W. & Ottosen, L. D. M. (2025). Exploring increased concentration of capture agent and methanogenic activity in Bio-Integrated Carbon Capture and Utilization (BICCU) reactors. Abstract ID: 4039 / Poster Number: 372.17P (770=. Poster session presented at FEMS MICRO 2025, Milan, Italy.

Wijesooriya, M. M., Kofoed, M. V. W., Ottosen, L. D. M., Nielsen, J. L. & Eskildsen, M. (2025). Exploring methanogen-carbon capture agent interactions in bio-integrated carbon capture and utilization (BICCU) into biomethane. O1A-002 (29). Abstract from The 5th International Conference on Biogas Microbiology, Galway, Ireland.

Ambrose, H. W., Dalby, F. R., Feilberg, A. & Kofoed, M. V. W. (2024). Chemical and biological surfactant treatments to mitigate methane emissions from manure pits in pig barns. Abstract from International symposium on gas and dust emissions from livestock (2024), Valencia, Spain.

Ambrose, H. W., Dalby, F. R., Feilberg, A. & Wegener Kofoed, M. V. (2024). Dose-dependent effects of sodium dodecyl sulfate and hydrogen peroxide treatments on methane emission from pig manure during storage. Journal of Environmental Management, 364, Article 121415. https://doi.org/10.1016/j.jenvman.2024.121415

Ambrose, H. W., Bambace, M. F., Marietou, A., Schwab, C., Kofoed, M. V. W. & Feilberg, A. (2024). Reuterin based biological treatment to mitigate methane emissions from pig slurry storage. Abstract from International symposium on gas and dust emissions from livestock (2024), Valencia, Spain.

Anand, M., Panigrahi, S., Kofoed, M. V. W., Aghababaei, R. & Agarwala, S. (2024). Bioinspired poly(vinyl alcohol) films with tunable adhesion and self-healing for biodegradable electronics and beyond. Sustainable Materials and Technologies, 41, Article e01084. https://doi.org/10.1016/j.susmat.2024.e01084

Engelbrecht, N., Sieborg, M. U., Ottosen, L. D. M. & Kofoed, M. V. W. (2024). Metabolic heat production impacts industrial upscaling of ex situ biomethanation trickle-bed reactors. Energy Conversion and Management, 299, Article 117769. https://doi.org/10.1016/j.enconman.2023.117769

Kirstine Hessellund Nielsen, A., Ditlev Mørck Ottosen, L. & Vedel Wegener Kofoed, M. (2024). Development of membrane bioreactor for conversion of flue Gas-CO₂ to C1 and C2 biomolecules. Chemical Engineering Journal, 499, Article 155780. https://doi.org/10.1016/j.cej.2024.155780

Kleikamp, H. B. C., Palacios, P. A., Kofoed, M. V. W., Papacharalampos, G., Bentien, A. & Nielsen, J. L. (2024). The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities. Environmental Science & Technology, 58(15), 6637-6646. https://doi.org/10.1021/acs.est.3c07725

Kundu, D., Samanta, P., Bhowmick, S., Panigrahi, S., Dey, S., Nath, K., Rajak, R. C., Sherpa, K. C., Barathi, A., Kofoed, M. V. W., Kumar, S. & Ki Lin, C. S. (2024). Heterogeneous catalysts for sustainable biofuel production: A paradigm shift towards renewable energy. Biocatalysis and Agricultural Biotechnology, 62, Article 103432. https://doi.org/10.1016/j.bcab.2024.103432

Oliveira, J. M. S., Ottosen, L. D. M. & Kofoed, M. V. W. (2024). Continuous biomethanation of flue gas-carbon dioxide using bio-integrated carbon capture and utilization. Bioresource Technology, 399, Article 130506. https://doi.org/10.1016/j.biortech.2024.130506

Palacios, P. A., Philips, J., Bentien, A. & Kofoed, M. V. W. (2024). Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications. Biotechnology Advances, 73, 108369. Article 108369. https://doi.org/10.1016/j.biotechadv.2024.108369

Sadegh, N., Uniacke, J., Feilberg, A. & Kofoed, M. V. W. (2024). Assessment of transient hydrogen sulfide peak emissions caused by biogas plant operation. Journal of Cleaner Production, 468, Article 142920. https://doi.org/10.1016/j.jclepro.2024.142920

Sieborg, M. U., Nielsen, A. K. H., Ottosen, L. D. M., Daasbjerg, K. & Kofoed, M. V. W. (2024). Bio-integrated carbon capture and utilization: at the interface between capture chemistry and archaeal CO₂ reduction. Nature Communications, 15(1), Article 7492. https://doi.org/10.1038/s41467-024-51700-3

Sieborg, M. U., Santos Oliveira, J. M., Ottosen, L. D. M. & Kofoed, M. V. W. (2024). Flue-to-fuel: Bio-integrated carbon capture and utilization of dilute carbon dioxide gas streams to renewable methane. Energy Conversion and Management, 302, Article 118090. https://doi.org/10.1016/j.enconman.2024.118090

Sieborg, M. U., Engelbrecht, N., Ottosen, L. D. M. & Kofoed, M. V. W. (2024). Sunshine-to-fuel: Demonstration of coupled photovoltaic-driven biomethanation operation, process, and techno-economical evaluation. Energy Conversion and Management, 299, Article 117767. https://doi.org/10.1016/j.enconman.2023.117767

Ambrose, H. W., Dalby, F. R., Feilberg, A. & Kofoed, M. V. W. (2023). Additives and methods for the mitigation of methane emission from stored liquid manure. Biosystems Engineering, 229, 209-245. https://doi.org/10.1016/j.biosystemseng.2023.03.015

Ambrose, H. W., Dalby, F. R., Feilberg, A. & Kofoed, M. V. W. (2023). Effects of surfactant, oxidant, and flocculant treatments on methane emission from pig slurry during storage. Journal of Cleaner Production, 430, Article 139665. https://doi.org/10.1016/j.jclepro.2023.139665

Ambrose, H. W., Dalby, F. R., Feilberg, A. & Kofoed, M. V. W. (2023). Mitigation of methane emission from pig slurry storage with sodium dodecyl sulfate and hydrogen peroxide treatments. 222. Poster session presented at RAMIRAN, Cambridge, United Kingdom.

Feilberg, A., Ambrose, H. W., Dalby, F. R. & Kofoed, M. V. W. (2023). Residual slurry treatment combined with weekly discharge: Elimination of in-house methane emission in livestock facilities. Abstract from Agri Food and Climate Circle 2023, København, Denmark.

Kofoed, M. V. W. & Mørck Ottosen, L. D. (2023). Mikroorganismer omdanner menneskeskab CO₂. Aktuel Naturvidenskab, 2023(4), 13-16. https://aktuelnaturvidenskab.dk/find-artikel/nyeste-numre/4-2023/mikroorganismer-omdanner-menneskeskabt-co2

Kuipers, S. B. & Kofoed, M. V. W. (2023). Biomethanation of syngas in packed bed reactors: A study of adaptation and microbial pathways in a thermophilic mixed consortium. Bioresource Technology Reports, 24, Article 101667. https://doi.org/10.1016/j.biteb.2023.101667

Palacios, P. A., Kleikamp, H., Nielsen, J. L., Jensen, M. B., Bentien, A. & Kofoed, M. V. W. (2023). Quinone-mediated extracellular electron transfer processes in ex situ biomethanation reactors. Bioresource Technology Reports, 24, Article 101671. https://doi.org/10.1016/j.biteb.2023.101671

Romio, C., Kofoed, M. V. W. & Møller, H. B. (2023). Exploring increased hydraulic retention time as a cost-efficient way of valorizing residual biogas potential. Bioresource Technology, 387, 129646. Article 129646. https://doi.org/10.1016/j.biortech.2023.129646

Sieborg, M. U., Ottosen, L. D. M. & Kofoed, M. V. W. (2023). Enhanced process control of trickle-bed reactors for biomethanation by vertical profiling directed by hydrogen microsensor monitoring. Bioresource Technology, 384, Article 129242. https://doi.org/10.1016/j.biortech.2023.129242

Dalby, F. R., Kofoed, M. V. W., Guldberg, L. B. & Feilberg, A. (2022). Acidification with inorganic and organic acids to reduce greenhouse gas emission from pig slurry: Acidification with inorganic and organic acids to reduce greenhouse gas emission from pig slurry. Abstract from 8th International Greenhouse Gas & Animal Agriculture Conference, Orlando, United States.

Dalby, F. R., Guldberg, L. B., Feilberg, A. & Kofoed, M. V. W. (2022). Reducing greenhouse gas emissions from pig slurry by acidification with organic and inorganic acids. PLoS One, 17(5), Article e0267693. https://doi.org/10.1371/journal.pone.0267693

de Jonge, N., Poulsen, J. S., Vechi, N. T., Kofoed, M. V. W. & Nielsen, J. L. (2022). Wood-Ljungdahl pathway utilisation during in situ H₂ biomethanation. Science of the Total Environment, 806(Part 3), Article 151254. https://doi.org/10.1016/j.scitotenv.2021.151254

Romio, C., Vedel Wegener Kofoed, M. & Bjarne Møller, H. (2022). Effect of ultrasonic and electrokinetic post-treatments on methane yield and viscosity of agricultural digestate. Bioresource Technology, 358, Article 127388. https://doi.org/10.1016/j.biortech.2022.127388

Ahmmed, M. S., Jensen, M. B., Kofoed, M. V. W., Ottosen, L. D. M. & Batstone, D. J. (2021). Hydrodynamic analysis of full-scale in-situ biogas upgrading in manure digesters. Water Research, 203, Article 117528. https://doi.org/10.1016/j.watres.2021.117528

Aryal, N., Ottosen, L. D. M., Bentien, A., Pant, D. & Kofoed, M. V. W. (2021). Bioelectrochemical systems for biogas upgrading and biomethane production. In N. Aryal, L. D. Mørck Ottosen, M. Vedel Wegener Kofoed & D. Pant (Eds.), Emerging Technologies and Biological Systems for Biogas Upgrading (pp. 363-382). Academic Press. https://doi.org/10.1016/B978-0-12-822808-1.00013-1

Aryal, N., Ottosen, L. D. M., Kofoed, M. V. W. & Pant, D. (Eds.) (2021). Emerging Technologies and Biological Systems for Biogas Upgrading. (1 ed.) Academic Press. https://doi.org/10.1016/B978-0-12-822808-1.00019-2

Dos Reis Vechi, N., Agnessens, L. M., Feilberg, A., Ottosen, L. D. M. & Kofoed, M. V. W. (2021). In situ biomethanation: Inoculum origin influences acetate consumption rate during hydrogen addition. Bioresource Technology Reports, 14, Article 100656. https://doi.org/10.1016/j.biteb.2021.100656

Jensen, M. B., de Jonge, N., Dolriis, M. D., Kragelund, C., Holst Fischer, C., Eskesen, M., Noer, K. W., Møller, H. B., Ottosen, L. D. M., Nielsen, J. L. & Kofoed, M. V. W. (2021). Cellulolytic and Xylanolytic Microbial Communities Associated With Lignocellulose-Rich Wheat Straw Degradation in Anaerobic Digestion. Frontiers in Microbiology, 12, Article 645174. https://doi.org/10.3389/fmicb.2021.645174

Jensen, M. B., Ottosen, L. D. M. & Kofoed, M. V. W. (2021). H₂ gas-liquid mass transfer: A key element in biological Power-to-Gas methanation. Renewable & Sustainable Energy Reviews, 147, Article 111209. https://doi.org/10.1016/j.rser.2021.111209

Jensen, M. B., Jensen, B., Ottosen, L. D. M. & Kofoed, M. V. W. (2021). Integrating H2 injection and reactor mixing for low-cost H2 gas-liquid mass transfer in full-scale in situ biomethanation. Biochemical Engineering Journal, 166, Article 107869. https://doi.org/10.1016/j.bej.2020.107869

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Michael Vedel Wegener Kofoed