Lecture by: AC-TAP Pernille Ommen Andersen and Postdoc Anne-Sofie R. Ballegaard
Title: ”Isolation of antigen-specific nanobodies by combining animal immunization with yeast surface display”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
In our laboratory we engineer and produce nanobodies towards different targets of interest in allergy.
In this presentation we will take you on the journey from animal immunization to in vitro and in vivo testing of identified nanobodies, and we will show how we use yeast surface display in the selection process.
Lecture by: Stephanie Descroix
Title: ”Developing organ on chip for biology and clinics”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
The development of a new generation of in vitro models is of interest in different fields such as basic research in life science to decipher physiological and patho-physiological mechanisms or in pharma companies to drastically improve drug screening process. In the last decade, basic research showed how our understanding of the behavior of physiological and patho-physiological systems can be greatly increased thanks to a rational simplification of the human body in artificial microsystems. Among these different models, organ on chips are considered today as key technologies.
In this talk, we will demonstrate how microfluidics and microfabrication can be used to develop new relevant in vitro models in particular a gut on chip model that allows to the study for the first time he interplay between, stromal and epithelial cells.
As a critical problem in the development and deployment of effective anti-cancer treatments remains the lack of adequate in-vitro model systems, in a second part, I will focus on tumor on chip development recapitulating in vitro the complexity of the tumor-microenvironment.
Doctor, Research Director CNRS, Institut Curie, Paris
Dr. Stéphanie Descroix is team leader of the MMBM team at Institut Curie Paris and Institut Pierre Gilles De Gennes. Her team is worldly renowned for development of microfluidic device and concepts for fundamental biology, biophysics and clinic, with a strong commitment to technology transfer in the field of medicine and life sciences. The MMBM team is cofounder of the Institut Pierre Gilles De Gennes for Microfluidics.
Lecture by: Catalina Suarez
Title: ”In silico study of the transport mechanisms of the blood brain barrier”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
This study presents the modelling of different transport mechanisms of molecules through the blood brain barrier in COMSOL, attempting to replicate experimental results found in literature to create a functional and accurate computational model to predict how particles will behave when crossing from the blood stream to the brain or viceversa.
Master Student
Lecture by: Hekla Liv Mariasdottir
Title: ”Blood-Brain Barrier Models in vitro: Image Analysis of Vesicular Trafficking and 3D Growth Guidance of Brain Endothelial Cells”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
The restrictive properties of the blood brain barrier (BBB) make drug delivery to the brain challenging. Questions related to the exact route of molecular transport across the BBB remain unanswered, however transferrin receptor (TfR)-mediated transcytosis through endothelial cells is believed to be a promising target for delivering therapeutics to the brain. I will present the master thesis I’m currently working on, focusing on super resolution microscopy of vesicular transport in endothelial cells, as well as mechanical studies of human induced pluripotent stem cell-derived brain endothelial cells grown on 3D scaffolds.
Master Student
Lecture by: Jens Vinge Nygaard
Title: ”EMBLs Mathematics for Life”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
I will report on the recent EMBL course Mathematics for Life detailing how biochemetry and pathways is modelled and used to describe cellular behavior. I will then focus on what is wrong with a perspective on cells that is solely based on chemistry and introduce the concept of mechanogeometry that we will explore in a upcoming new project funded by a VILLUM Synergy grant between mathematics and BCE at AU.
Lecture by: Christoph Alexander Müller
Title: ”Optoresponsive particles for intracellular electrical interrogation”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Wireless electrical modulation of cells via opto-stimulation is an emerging approach to trigger and control bioelectric phenomena with high spatiotemporal resolution. In my talk, I will show how optoresponsive materials like graphitic carbon nitride can be used to control neural growth, and act as a tool for intracellular electrical interrogation.
Lecture by: Mikkel Haarslev S. Marqvorsen
Title: ”From Glycosylation to Immunology - A brief tour of miscellaneous projects”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Mikkel Marqvorsen is a newly appointed teaching assistant professor at BCE. In this talk, Mikkel will provide a brief overview of the various fields in which he has worked during the course of a ph.d and two postdoc positions before starting at BCE in June. Mikkel started as a ph.d. student working on glycosylation chemistry at the Dept. of Chemistry (AU) and then worked on the interface between organic chemistry and cell biology (Chemical Biology) in Leiden (NL) for three years before returning to AU at the Dept. of Biomedicine to focus on Immune Cell Biology.
Lecture by: Simon Sutter Rolighed
Title: ”Enriching Synaptosomes for Imaging”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Synaptosomes are isolated synaptic terminals from neurons obtained by homogenization of brain tissue followed by fractionation using Percoll density gradient centrifugation. We have found that these samples contain a variety of vesicular structures, making the search and identification of synaptosomes a challenging and time-consuming task. This presentation will be part two of my master's thesis on the use of immunomagnetic isolation for selective enrichment of synaptosomes.
Lecture by: Josephine Baunvig Aagaard
Title: ”Nanobody-based inhibitors of allergen-mediated anaphylaxis”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Hymenoptera venom allergy is one of the most common causes of anaphylaxis. Central in the allergic reaction is the binding of venom allergens to IgE/receptor complexes on the surface of effector cells triggering immediate allergic reactions and potentially anaphylaxis. In my PhD project, I have developed and characterized nanobodies and nanobody-based formats that block the IgE/allergen interaction and thereby potentially reduce the risk of anaphylaxis during allergen exposure.
Lecture by: Peter Johansen
Title: ”Experimental Cardiovascular Biomechanics”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
This talk gives an overview of ongoing projects in CAVE Lab. Common for all projects are that they are anchored in clinical problems and challenges and therefore conducted in close collaboration with cardiac surgeons and interventional cardiologists. The general approach is to create in vitro models that can mimic the physiology surrounding different devices, implants, and procedures.
Lecture by: Thomas Lykke-Møller Sørensen
Title: ”Highlight(s) from EMBO/EMBL symposium on Mechanobiology”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Lecture by: Frederik Høbjerg Svejsø
Title: ”Anti-EpCAM Conjugated Melt-Electrowritten Microfibrous Filter for Capturing and Culturing Circulating Tumor Cells”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
This presentation will focus on my Master's thesis, where I am utilizing melt-electrowriting to fabricate porous filters of polycaprolactone (PCL). The fiber surface is subsequently conjugated with an anti-EpCAM antibody to allow for affinity-based capture of rare circulating tumor cells (CTCs) from a liquid biopsy, i.e. a blood sample. Due to the biocompatibility of PCL, the caught CTCs will be cultured ex vivo directly on the filter and the formed clusters may contain information useful for personalized medicine.
Master Student
Lecture by: Pernille Ommen Andersen
Title: ”Development of a targeted drug delivery system for Staphylococcus aureus biofilm infections”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Conventional antibiotic treatment is inadequate for eradication of bacterial biofilm infections as only sublethal concentrations can be administered safely to patients. A potential solution to this challenge is targeted drug delivery.
In my PhD study, we tailored an aptamer-targeted liposomal drug delivery system that accumulates in S. aureus biofilm and delivers a combination of antibiotics locally inside the biofilm. In this presentation I will show the results and discuss what we learned from the study.
Lecture by: Peter Johansen
Title: ”Experimental Cardiovascular Biomechanics”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
This talk gives an overview of ongoing projects in CAVE Lab. Common for all projects are that they are anchored in clinical problems and challenges and therefore conducted in close collaboration with cardiac surgeons and interventional cardiologists. The general approach is to create in vitro models that can mimic the physiology surrounding different devices, implants, and procedures.
Lecture by: Camilla Jessen
Title: ”Establishment and Characterization of Polymicrobial Inter-kingdom three-species biofilm models mimicking the microenvironment of In Vivo Chronic Wounds in Diabetic Foot Ulcers”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Chronic wounds have become a global health problem. Chronic wounds are often infected with polymicrobial biofilms that hinders the normal healing of wounds. Consequently, chronic wounds remain in the inflammatory phase of healing. In vitro studies of chronic wounds often utilizes simple, monocultural models that exhibit unrealistic fast eradication of biofilms, once treated with antimicrobials. Given that these results are not observed in the clinic, there has become a need for more complex in vitro models comprised of multiple microbes. During my thesis, i aim to establish a polymicrobial, inter-kingdom biofilm model comprised of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans.
Master Student
Lecture by: Alice Le Friec
Title: ”Cellularized biomaterials for neural tissue engineering”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
I will first discuss cell and biomaterial co-transplantation for regenerative medicine in the Central Nervous System. I will then introduce my latest research, which aims to design a cryopreservable cellularized scaffold for neural tissue repair.
Lecture by: Anne-Sofie Ravn Ballegaard
Title: ”Food allergy sensitization – knowledge from animal studies”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
The presentation will give an overview of food allergy and different routes of sensitization. This will include results from animal studies with a focus on intestinal permeability and immune responses.
Lecture by: Simon Sutter Rolighed
Title: ”Enriching synaptosomes for imaging”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Synaptosomes are isolated synaptic terminals from neurons which are obtained by homogenization of brain tissue followed by fractionation using Percoll density gradient centrifugation. We have found that these samples contain a variety of vesicular structures making the search and identification of synaptosomes challenging and time-consuming task. I’ll discuss our efforts to selective enrich the sample for synaptosomes.
Lecture by: Naija From Munk-Pedersen
Title: “Development of new mri protocol for knee osteoarthritis”
Place: Gustav Wieds Vej 10C, 8000 Aarhus C, Building 3130, Room 303.
Lecture by: Nele Van Dessel, CEO from Ernest Pharmaceuticals (USA)
Title: "The use of Salmonella bacteria to treat cancer”
Place: Zoom, https://aarhusuniversity.zoom.us/j/62375855091
The microbial biotechnology course will host an online guest lecture on the use of Salmonella bacteria to treat cancer.
The guest speaker is Nele Van Dessel, CEO from Ernest Pharmaceuticals (USA), a small startup company, trying to bring this new and intriguing microbial biotechnology to the market.
