Publications

All publications

Lara, A. R., Andersen, M. B., Madsen, A. A. V., Fønss, K. G., Irla, M., Taymaz-Nikerel, H., Martínez, L. M., Dicke, M. D., Mann, M., Magnus, J. B. & Gosset, G. (2025). Biomanufacturing Potential of Streamlined Cells. Biotechnology and Bioengineering, 122(12), 3309-3318. https://doi.org/10.1002/bit.70070

Pohlen, L., García, E., Martínez, L. M., Flores, N., Büchs, J., Gosset, G. & Lara, A. R. (2025). Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression. Biotechnology and Bioengineering, 122(6), 1598-1607. https://doi.org/10.1002/bit.28958

Bravo-Olín, J., Martínez-Carreón, S. A., Francisco-Solano, E., Lara, A. R. & Beltran-Vargas, N. E. (2024). Analysis of the role of perfusion, mechanical, and electrical stimulation in bioreactors for cardiac tissue engineering. Bioprocess and Biosystems Engineering, 47(6), 767-839. https://doi.org/10.1007/s00449-024-03004-5

de la Cruz, M., Kunert, F., Taymaz-Nikerel, H., Sigala, J.-C., Gosset, G., Büchs, J. & Lara, A. R. (2024). Increasing the Pentose Phosphate Pathway Flux to Improve Plasmid DNA Production in Engineered E. coli. Microorganisms, 12(1), Article 150. https://doi.org/10.3390/microorganisms12010150

Lara, A. R., Utrilla, J., Martínez, L. M., Krausch, N., Kaspersetz, L., Hidalgo, D., Cruz-Bournazou, N., Neubauer, P., Sigala, J. C., Gosset, G. & Büchs, J. (2024). Recombinant protein expression in proteome-reduced cells under aerobic and oxygen-limited regimes. Biotechnology and Bioengineering, 121(4), 1216-1230. https://doi.org/10.1002/bit.28645

Lara, A. R., Kunert, F., Vandenbroucke, V., Taymaz-Nikerel, H., Martínez, L. M., Sigala, J. C., Delvigne, F., Gosset, G. & Büchs, J. (2024). Transport-controlled growth decoupling for self-induced protein expression with a glycerol-repressible genetic circuit. Biotechnology and Bioengineering, 121(6), 1789-1802. https://doi.org/10.1002/bit.28697

Islas, F., Sabido, A., Sigala, J. C. & Lara, A. R. (2023). Design of microaerobically inducible miniR1 plasmids. mLife, 2(1), 101-104. https://doi.org/10.1002/mlf2.12058

Krausch, N., Kaspersetz, L., Gaytán-Castro, R. D., Schermeyer, M. T., Lara, A. R., Gosset, G., Cruz Bournazou, M. N. & Neubauer, P. (2023). Model-Based Characterization of E. coli Strains with Impaired Glucose Uptake. Bioengineering, 10(7), Article 808. https://doi.org/10.3390/bioengineering10070808

Fragoso-Jiménez, J. C., Gutierrez-Rios, R. M., Flores, N., Martinez, A., Lara, A. R., Delvigne, F. & Gosset, G. (2022). Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories. Microbial Cell Factories, 21(1), Article 189. https://doi.org/10.1186/s12934-022-01909-y

Velazquez, D., Sigala, J. C., Martínez, L. M., Gaytán, P., Gosset, G. & Lara, A. R. (2022). Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains. Microbial Cell Factories, 21(1), Article 183. https://doi.org/10.1186/s12934-022-01906-1

Taymaz-Nikerel, H. & Lara, A. R. (2022). Vitreoscilla haemoglobin: A tool to reduce overflow metabolism. Microorganisms, 10(1), Article 43. https://doi.org/10.3390/microorganisms10010043

Juárez, M., González-De La Rosa, C. H., Sigala, J. C. & Lara, A. R. (2021). Effect of vitreoscilla hemoglobin on recombinant protein expression and energy and redox state of cho cells. Revista Mexicana de Ingeniera Quimica, 20(1), 281-288. https://doi.org/10.24275/rmiq/Bio1866

Jaén, K. E., Velazquez, D., Sigala, J. C. & Lara, A. R. (2021). Enhancing microaerobic plasmid DNA production by chromosomal expression of Vitreoscilla hemoglobin in E. coli. Biochemical Engineering Journal, 166, Article 107862. https://doi.org/10.1016/j.bej.2020.107862

Arteaga, J. E., Cerros, K., Rivera-Becerril, E., Lara, A. R., Le Borgne, S. & Sigala, J. C. (2021). Furfural biotransformation in Acinetobacter baylyi ADP1 and Acinetobacter schindleri ACE. Biotechnology Letters , 43(5), 1043-1050. https://doi.org/10.1007/s10529-021-03094-1

Velazquez, D., Jaén, K. E., Sigala, J. C. & Lara, A. R. (2021). Minimized backbone and novel microaerobic promoters boost plasmid DNA production. Process Biochemistry, 106, 130-136. https://doi.org/10.1016/j.procbio.2021.04.013

Lara, A. R., Galindo, J., Jaén, K. E., Juárez, M. & Sigala, J. C. (2020). Physiological response of escherichia coli W3110 and BL21 to the aerobic expression of vitreoscilla hemoglobin. Journal of Microbiology and Biotechnology, 30(10), 1592-1596. https://doi.org/10.4014/JMB.2004.04030

de la Cruz, M., Ramírez, E. A., Sigala, J. C., Utrilla, J. & Lara, A. R. (2020). Plasmid DNA production in proteome-reduced Escherichia coli. Microorganisms, 8(9), 1-8. Article 1444. https://doi.org/10.3390/microorganisms8091444

Jaén, K. E., Velázquez, D., Sigala, J. C. & Lara, A. R. (2019). Design of a microaerobically inducible replicon for high-yield plasmid DNA production. Biotechnology and Bioengineering, 116(10), 2514-2525. https://doi.org/10.1002/bit.27091

Lara, A. R., Velázquez, D., Penella, I., Islas, F., González-De la Rosa, C. H. & Sigala, J. C. (2019). Design of a synthetic miniR1 plasmid and its production by engineered Escherichia coli. Bioprocess and Biosystems Engineering, 42(8), 1391-1397. https://doi.org/10.1007/s00449-019-02129-2

Lara, A. R., Jaén, K. E., Folarin, O., Keshavarz-Moore, E. & Büchs, J. (2019). Effect of the oxygen transfer rate on oxygen-limited production of plasmid DNA by Escherichia coli. Biochemical Engineering Journal, 150, Article 107303. https://doi.org/10.1016/j.bej.2019.107303

Jaén, K. E., Velazquez, D., Delvigne, F., Sigala, J. C. & Lara, A. R. (2019). Engineering E. coli for improved microaerobic pDNA production. Bioprocess and Biosystems Engineering, 42(9), 1457-1466. https://doi.org/10.1007/s00449-019-02142-5

Grijalva-Hernández, F., Vega-Estrada, J., Escobar-Rosales, M., Ortega-López, J., Aguilar-López, R., Lara, A. R. & Montes-Horcasitas, M. D. C. (2019). High kanamycin concentration as another stress factor additional to temperature to increase pdna production in e. Coli dh5α batch and fed-batch cultures. Microorganisms, 7(12), Article 711. https://doi.org/10.3390/microorganisms7120711

Lara, A. R. & Gosset, G. (2019). Minimal cells: Design, construction, biotechnological applications. Springer International Publishing. https://doi.org/10.1007/978-3-030-31897-0

Sigala, J. C., Quiroz, L., Arteaga, E., Olivares, R., Lara, A. R. & Martinez, A. (2019). Physiological and transcriptional comparison of acetate catabolism between Acinetobacter schindleri ACE and Escherichia coli JM101. FEMS Microbiology Letters, 366(12), Article fnz151. https://doi.org/10.1093/femsle/fnz151

Lara, A. R. & Gosset, G. (2019). Preface. In Minimal Cells: Design, Construction, Biotechnological Applications (pp. v-vi). Springer International Publishing.

Martínez, J. A., Rodriguez, A., Moreno, F., Flores, N., Lara, A. R., Ramírez, O. T., Gosset, G. & Bolivar, F. (2018). Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production. BMC Systems Biology, 12(1), Article 102. https://doi.org/10.1186/s12918-018-0632-4

Juárez, M., González-De la Rosa, C. H., Memún, E., Sigala, J. C. & Lara, A. R. (2017). Aerobic expression of Vitreoscilla hemoglobin improves the growth performance of CHO-K1 cells. Biotechnology Journal, 12(3), Article 1600438. https://doi.org/10.1002/biot.201600438

Lara, A. R., Jaén, K. E., Sigala, J. C., Mühlmann, M., Regestein, L. & Büchs, J. (2017). Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli. ACS Synthetic Biology, 6(2), 344-356. https://doi.org/10.1021/acssynbio.6b00233

Lara, A. R., Jaén, K. E., Sigala, J. C., Regestein, L. & Büchs, J. (2017). Evaluation of microbial globin promoters for oxygen-limited processes using Escherichia coli. Journal of Biological Engineering, 11(1), Article 39. https://doi.org/10.1186/s13036-017-0082-3

Sigala, J. C., Suárez, B. P., Lara, A. R., Borgne, S. L., Bustos, P., Santamaría, R. I., González, V. & Martinez, A. (2017). Genomic and physiological characterization of a laboratory-isolated Acinetobacter schindleri ACE strain that quickly and efficiently catabolizes acetate. Microbiology (United Kingdom), 163(7), 1052-1064. https://doi.org/10.1099/mic.0.000488

Jaén, K. E., Sigala, J. C., Olivares-Hernández, R., Niehaus, K. & Lara, A. R. (2017). Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli. BMC Biotechnology, 17(1), Article 60. https://doi.org/10.1186/s12896-017-0378-x

Taymaz-Nikerel, H. & Lara, A. R. (2016). Editorial: Quantitative systems biology for engineering organisms and pathways. Frontiers in Bioengineering and Biotechnology, 4(MAR), Article 22. https://doi.org/10.3389/fbioe.2016.00022

Ramírez, E. A., Velázquez, D. & Lara, A. R. (2016). Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli. Biotechnology Letters , 38(4), 651-657. https://doi.org/10.1007/s10529-015-2017-8

Galindo, J., Barrón, B. L. & Lara, A. R. (2016). Improved production of large plasmid DNA by enzyme-controlled glucose release. Annals of Microbiology, 66(3), 1337-1342. https://doi.org/10.1007/s13213-016-1218-2

Baert, J., Delepierre, A., Telek, S., Fickers, P., Toye, D., Delamotte, A., Lara, A. R., Jaén, K. E., Gosset, G., Jensen, P. R. & Delvigne, F. (2016). Microbial population heterogeneity versus bioreactor heterogeneity: Evaluation of Redox Sensor Green as an exogenous metabolic biosensor. Engineering in Life Sciences, 16(7), 643-651. https://doi.org/10.1002/elsc.201500149

Cortés, J. T., Flores, N., Bolívar, F., Lara, A. R. & Ramírez, O. T. (2016). Physiological effects of pH gradients on Escherichia coli during plasmid DNA production. Biotechnology and Bioengineering, 113(3), 598-611. https://doi.org/10.1002/bit.25817

Lara, A. R., Palomares, L. A. & Ramírez, O. T. (2016). Scale-down: Simulating large-scale cultures in the laboratory. In Industrial Biotechnology: Products and Processes (pp. 55-79). Wiley-VCH Verlag GmbH. https://doi.org/10.1002/9783527807833

Pablos, T. E., Olivares, R., Sigala, J. C., Ramírez, O. T. & Lara, A. R. (2016). Toward efficient microaerobic processes using engineered Escherichia coli W3110 strains. Engineering in Life Sciences, 16(7), 588-597. https://doi.org/10.1002/elsc.201500129

Oliveira, P. H., Mairhofer, J., Alves, P. M., Lara, A. R. & Kontoravdi, C. (2015). Advances in the development of biotherapeutics. BioMed Research International, 2015, Article 793876. https://doi.org/10.1155/2015/793876

Freudenau, I., Lutter, P., Baier, R., Schleef, M., Bednarz, H., Lara, A. R. & Niehaus, K. (2015). ColE1-plasmid production in Escherichia coli: Mathematical simulation and experimental validation. Frontiers in Bioengineering and Biotechnology, 3(SEP), Article 127. https://doi.org/10.3389/fbioe.2015.00127

Mairhofer, J. & Lara, A. R. (2014). Advances in host and vector development for the production of plasmid DNA vaccines. Methods in Molecular Biology, 1139, 505-541. https://doi.org/10.1007/978-1-4939-0345-0_38

Pablos, T. E., Sigala, J. C., Le Borgne, S. & Lara, A. R. (2014). Aerobic expression of Vitreoscilla hemoglobin efficiently reduces overflow metabolism in Escherichia coli. Biotechnology Journal, 9(6), 791-799. https://doi.org/10.1002/biot.201300388

Beltran, N. E., Reyes, A. & Lara, A. R. (2014). Cardiac tissue engineering as an alternative to current therapies: Economical and technical challenges. Experimental and Clinical Cardiology, 20(8), 3375-3388.

Gálvez, R. M., Pablos, T. E., Sigala, J. C. & Lara, A. R. (2014). Co-utilization of glucose and xylose increases growth rate without affecting plasmid dna yield of engineered E. coli. Revista Mexicana de Ingeniera Quimica, 13(2), 387-391.

Wunderlich, M., Taymaz-Nikerel, H., Gosset, G., Ramírez, O. T. & Lara, A. R. (2014). Effect of growth rate on plasmid DNA production and metabolic performance ofengineered Escherichia coli strains. Journal of Bioscience and Bioengineering, 117(3), 336-342. https://doi.org/10.1016/j.jbiosc.2013.08.007

Licona-Cassani, C., Lara, A. R., Cabrera-Valladares, N., Escalante, A., Hernández-Chávez, G., Martinez, A., Bolívar, F. & Gosset, G. (2014). Inactivation of pyruvate kinase or the phosphoenolpyruvate: Sugar phosphotransferase system increases shikimic and dehydroshikimic acid yields from glucose in bacillus subtilis. Journal of Molecular Microbiology and Biotechnology, 24(1), 37-45. https://doi.org/10.1159/000355264

Delvigne, F., Zune, Q., Lara, A. R., Al-Soud, W. & Sørensen, S. J. (2014). Metabolic variability in bioprocessing: Implications of microbial phenotypic heterogeneity. Trends in Biotechnology, 32(12), 608-616. https://doi.org/10.1016/j.tibtech.2014.10.002

Jaén, K. E., Lara, A. R. & Ramírez, O. T. (2013). Effect of heating rate on pDNA production by E. coli. Biochemical Engineering Journal, 79, 230-238. https://doi.org/10.1016/j.bej.2013.08.006

Caspeta, L., Lara, A. R., Pérez, N. O., Flores, N., Bolívar, F. & Ramírez, O. T. (2013). Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies. Journal of Biotechnology, 167(1), 47-55. https://doi.org/10.1016/j.jbiotec.2013.06.001

Fuentes, L. G., Lara, A. R., Martínez, L. M., Ramírez, O. T., Martínez, A., Bolívar, F. & Gosset, G. (2013). Modification of glucose import capacity in Escherichia coli: Physiologic consequences and utility for improving DNA vaccine production. Microbial Cell Factories, 12(1), Article 42. https://doi.org/10.1186/1475-2859-12-42

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Revised 16.01.2025