Publications

All publications

Lara, A. R. & Gosset, G. (2019). Preface. In Minimal Cells: Design, Construction, Biotechnological Applications (pp. v-vi). Springer International Publishing.

Lara, A. R. & Ramírez, O. T. (2012). Plasmid DNA production for therapeutic applications. In A. Lorence (Ed.), Recombinant Gene Expression: Reviews and Protocols, Third Edition (pp. 271-303) https://doi.org/10.1007/978-1-61779-433-9_14

Lara, A. R., Palomares, L. A. & Ramírez, O. T. (2016). Scale-down: Simulating large-scale cultures in the laboratory. In Industrial Biotechnology: Products and Processes (pp. 55-79). Wiley-VCH Verlag GmbH. https://doi.org/10.1002/9783527807833

Lara, A. R. & Gosset, G. (2019). Minimal cells: Design, construction, biotechnological applications. Springer International Publishing. https://doi.org/10.1007/978-3-030-31897-0

Oliveira, P. H., Mairhofer, J., Alves, P. M., Lara, A. R. & Kontoravdi, C. (2015). Advances in the development of biotherapeutics. BioMed Research International, 2015, Article 793876. https://doi.org/10.1155/2015/793876

Taymaz-Nikerel, H. & Lara, A. R. (2016). Editorial: Quantitative systems biology for engineering organisms and pathways. Frontiers in Bioengineering and Biotechnology, 4(MAR), Article 22. https://doi.org/10.3389/fbioe.2016.00022

Bravo-Olín, J., Martínez-Carreón, S. A., Francisco-Solano, E., Lara, A. R. & Beltran-Vargas, N. E. (2024). Analysis of the role of perfusion, mechanical, and electrical stimulation in bioreactors for cardiac tissue engineering. Bioprocess and Biosystems Engineering, 47(6), 767-839. https://doi.org/10.1007/s00449-024-03004-5

Beltran, N. E., Reyes, A. & Lara, A. R. (2014). Cardiac tissue engineering as an alternative to current therapies: Economical and technical challenges. Experimental and Clinical Cardiology, 20(8), 3375-3388.

Lara, A. R., Galindo, E., Ramírez, O. T. & Palomares, L. A. (2006). Living with heterogeneities in bioreactors: Understanding the effects of environmental gradients on cells. Molecular Biotechnology, 34(3), 355-381. https://doi.org/10.1385/MB:34:3:355

Delvigne, F., Zune, Q., Lara, A. R., Al-Soud, W. & Sørensen, S. J. (2014). Metabolic variability in bioprocessing: Implications of microbial phenotypic heterogeneity. Trends in Biotechnology, 32(12), 608-616. https://doi.org/10.1016/j.tibtech.2014.10.002

Lara, A. R. (2011). Recombinant protein production in escherichia coli. Revista Mexicana de Ingeniera Quimica, 10(2), 209-223.

Lara, A. R., Jaén, K. E., Sigala, J. C., Regestein, L. & Büchs, J. (2017). Evaluation of microbial globin promoters for oxygen-limited processes using Escherichia coli. Journal of Biological Engineering, 11(1), Article 39. https://doi.org/10.1186/s13036-017-0082-3

Mairhofer, J. & Lara, A. R. (2014). Advances in host and vector development for the production of plasmid DNA vaccines. Methods in Molecular Biology, 1139, 505-541. https://doi.org/10.1007/978-1-4939-0345-0_38

Pablos, T. E., Sigala, J. C., Le Borgne, S. & Lara, A. R. (2014). Aerobic expression of Vitreoscilla hemoglobin efficiently reduces overflow metabolism in Escherichia coli. Biotechnology Journal, 9(6), 791-799. https://doi.org/10.1002/biot.201300388

Juárez, M., González-De la Rosa, C. H., Memún, E., Sigala, J. C. & Lara, A. R. (2017). Aerobic expression of Vitreoscilla hemoglobin improves the growth performance of CHO-K1 cells. Biotechnology Journal, 12(3), Article 1600438. https://doi.org/10.1002/biot.201600438

Lara, A. R., Andersen, M. B., Madsen, A. A. V., Fønss, K. G., Irla, M., Taymaz-Nikerel, H., Martínez, L. M., Dicke, M. D., Mann, M., Magnus, J. B. & Gosset, G. (2025). Biomanufacturing Potential of Streamlined Cells. Biotechnology and Bioengineering, 122(12), 3309-3318. https://doi.org/10.1002/bit.70070

Lara, A. R., Jaén, K. E., Sigala, J. C., Mühlmann, M., Regestein, L. & Büchs, J. (2017). Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli. ACS Synthetic Biology, 6(2), 344-356. https://doi.org/10.1021/acssynbio.6b00233

Freudenau, I., Lutter, P., Baier, R., Schleef, M., Bednarz, H., Lara, A. R. & Niehaus, K. (2015). ColE1-plasmid production in Escherichia coli: Mathematical simulation and experimental validation. Frontiers in Bioengineering and Biotechnology, 3(SEP), Article 127. https://doi.org/10.3389/fbioe.2015.00127

Lara, A. R., Knabben, I., Regestein, L., Sassi, J., Caspeta, L., Ramírez, O. T. & Büchs, J. (2011). Comparison of oxygen enriched air vs. pressure cultivations to increase oxygen transfer and to scale-up plasmid DNA production fermentations. Engineering in Life Sciences, 11(4), 382-386. https://doi.org/10.1002/elsc.201000104

Gálvez, R. M., Pablos, T. E., Sigala, J. C. & Lara, A. R. (2014). Co-utilization of glucose and xylose increases growth rate without affecting plasmid dna yield of engineered E. coli. Revista Mexicana de Ingeniera Quimica, 13(2), 387-391.

Jaén, K. E., Velázquez, D., Sigala, J. C. & Lara, A. R. (2019). Design of a microaerobically inducible replicon for high-yield plasmid DNA production. Biotechnology and Bioengineering, 116(10), 2514-2525. https://doi.org/10.1002/bit.27091

Lara, A. R., Velázquez, D., Penella, I., Islas, F., González-De la Rosa, C. H. & Sigala, J. C. (2019). Design of a synthetic miniR1 plasmid and its production by engineered Escherichia coli. Bioprocess and Biosystems Engineering, 42(8), 1391-1397. https://doi.org/10.1007/s00449-019-02129-2

Islas, F., Sabido, A., Sigala, J. C. & Lara, A. R. (2023). Design of microaerobically inducible miniR1 plasmids. mLife, 2(1), 101-104. https://doi.org/10.1002/mlf2.12058

Wunderlich, M., Taymaz-Nikerel, H., Gosset, G., Ramírez, O. T. & Lara, A. R. (2014). Effect of growth rate on plasmid DNA production and metabolic performance ofengineered Escherichia coli strains. Journal of Bioscience and Bioengineering, 117(3), 336-342. https://doi.org/10.1016/j.jbiosc.2013.08.007

Jaén, K. E., Lara, A. R. & Ramírez, O. T. (2013). Effect of heating rate on pDNA production by E. coli. Biochemical Engineering Journal, 79, 230-238. https://doi.org/10.1016/j.bej.2013.08.006

Lara, A. R., Jaén, K. E., Folarin, O., Keshavarz-Moore, E. & Büchs, J. (2019). Effect of the oxygen transfer rate on oxygen-limited production of plasmid DNA by Escherichia coli. Biochemical Engineering Journal, 150, Article 107303. https://doi.org/10.1016/j.bej.2019.107303

Juárez, M., González-De La Rosa, C. H., Sigala, J. C. & Lara, A. R. (2021). Effect of vitreoscilla hemoglobin on recombinant protein expression and energy and redox state of cho cells. Revista Mexicana de Ingeniera Quimica, 20(1), 281-288. https://doi.org/10.24275/rmiq/Bio1866

Jaén, K. E., Velazquez, D., Delvigne, F., Sigala, J. C. & Lara, A. R. (2019). Engineering E. coli for improved microaerobic pDNA production. Bioprocess and Biosystems Engineering, 42(9), 1457-1466. https://doi.org/10.1007/s00449-019-02142-5

Lara, A. R., Vazquez-Limón, C., Gosset, G., Bolívar, F., López-Munguía, A. & Ramírez, O. T. (2006). Engineering Escherichia coli to improve culture performance and reduce formation of by-products during recombinant protein production under transient intermittent anaerobic conditions. Biotechnology and Bioengineering, 94(6), 1164-1175. https://doi.org/10.1002/bit.20954

Borja, G. M., Meza Mora, E., Barrón, B., Gosset, G., Ramírez, O. T. & Lara, A. R. (2012). Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode. Microbial Cell Factories, 11, Article 132. https://doi.org/10.1186/1475-2859-11-132

Ramírez, E. A., Velázquez, D. & Lara, A. R. (2016). Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli. Biotechnology Letters , 38(4), 651-657. https://doi.org/10.1007/s10529-015-2017-8

Pablos, T. E., Soto, R., Mora, E. M., Le Borgne, S., Ramírez, O. T., Gosset, G. & Lara, A. R. (2012). Enhanced production of plasmid DNA by engineered Escherichia coli strains. Journal of Biotechnology, 158(4), 211-214. https://doi.org/10.1016/j.jbiotec.2011.04.015

Jaén, K. E., Velazquez, D., Sigala, J. C. & Lara, A. R. (2021). Enhancing microaerobic plasmid DNA production by chromosomal expression of Vitreoscilla hemoglobin in E. coli. Biochemical Engineering Journal, 166, Article 107862. https://doi.org/10.1016/j.bej.2020.107862

Caspeta, L., Lara, A. R., Pérez, N. O., Flores, N., Bolívar, F. & Ramírez, O. T. (2013). Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies. Journal of Biotechnology, 167(1), 47-55. https://doi.org/10.1016/j.jbiotec.2013.06.001

Lara, A. R., Taymaz-Nikerel, H., Mashego, M. R., Van Gulik, W. M., Heijnen, J. J., Ramírez, O. T. & Van Winden, W. A. (2009). Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses. Biotechnology and Bioengineering, 104(6), 1153-1161. https://doi.org/10.1002/bit.22503

Arteaga, J. E., Cerros, K., Rivera-Becerril, E., Lara, A. R., Le Borgne, S. & Sigala, J. C. (2021). Furfural biotransformation in Acinetobacter baylyi ADP1 and Acinetobacter schindleri ACE. Biotechnology Letters , 43(5), 1043-1050. https://doi.org/10.1007/s10529-021-03094-1

Sigala, J. C., Suárez, B. P., Lara, A. R., Borgne, S. L., Bustos, P., Santamaría, R. I., González, V. & Martinez, A. (2017). Genomic and physiological characterization of a laboratory-isolated Acinetobacter schindleri ACE strain that quickly and efficiently catabolizes acetate. Microbiology (United Kingdom), 163(7), 1052-1064. https://doi.org/10.1099/mic.0.000488

Fragoso-Jiménez, J. C., Gutierrez-Rios, R. M., Flores, N., Martinez, A., Lara, A. R., Delvigne, F. & Gosset, G. (2022). Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories. Microbial Cell Factories, 21(1), Article 189. https://doi.org/10.1186/s12934-022-01909-y

Velazquez, D., Sigala, J. C., Martínez, L. M., Gaytán, P., Gosset, G. & Lara, A. R. (2022). Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains. Microbial Cell Factories, 21(1), Article 183. https://doi.org/10.1186/s12934-022-01906-1

Jaén, K. E., Sigala, J. C., Olivares-Hernández, R., Niehaus, K. & Lara, A. R. (2017). Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli. BMC Biotechnology, 17(1), Article 60. https://doi.org/10.1186/s12896-017-0378-x

Soto, R., Caspeta, L., Barrón, B., Gosset, G., Ramírez, O. T. & Lara, A. R. (2011). High cell-density cultivation in batch mode for plasmid DNA production by a metabolically engineered E. coli strain with minimized overflow metabolism. Biochemical Engineering Journal, 56(3), 165-171. https://doi.org/10.1016/j.bej.2011.06.003

Knabben, I., Regestein, L., Marquering, F., Steinbusch, S., Lara, A. R. & Büchs, J. (2010). High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. Journal of Biotechnology, 150(1), 73-79. https://doi.org/10.1016/j.jbiotec.2010.07.006

Grijalva-Hernández, F., Vega-Estrada, J., Escobar-Rosales, M., Ortega-López, J., Aguilar-López, R., Lara, A. R. & Montes-Horcasitas, M. D. C. (2019). High kanamycin concentration as another stress factor additional to temperature to increase pdna production in e. Coli dh5α batch and fed-batch cultures. Microorganisms, 7(12), Article 711. https://doi.org/10.3390/microorganisms7120711

Galindo, J., Barrón, B. L. & Lara, A. R. (2016). Improved production of large plasmid DNA by enzyme-controlled glucose release. Annals of Microbiology, 66(3), 1337-1342. https://doi.org/10.1007/s13213-016-1218-2

Licona-Cassani, C., Lara, A. R., Cabrera-Valladares, N., Escalante, A., Hernández-Chávez, G., Martinez, A., Bolívar, F. & Gosset, G. (2014). Inactivation of pyruvate kinase or the phosphoenolpyruvate: Sugar phosphotransferase system increases shikimic and dehydroshikimic acid yields from glucose in bacillus subtilis. Journal of Molecular Microbiology and Biotechnology, 24(1), 37-45. https://doi.org/10.1159/000355264

de la Cruz, M., Kunert, F., Taymaz-Nikerel, H., Sigala, J.-C., Gosset, G., Büchs, J. & Lara, A. R. (2024). Increasing the Pentose Phosphate Pathway Flux to Improve Plasmid DNA Production in Engineered E. coli. Microorganisms, 12(1), Article 150. https://doi.org/10.3390/microorganisms12010150

Chávez-Béjar, M. I., Lara, A. R., López, H., Hernández-Chávez, G., Martinez, A., Ramírez, O. T., Bolívar, F. & Gosset, G. (2008). Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilis. Applied and Environmental Microbiology, 74(10), 3284-3290. https://doi.org/10.1128/AEM.02456-07

Martínez, J. A., Rodriguez, A., Moreno, F., Flores, N., Lara, A. R., Ramírez, O. T., Gosset, G. & Bolivar, F. (2018). Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production. BMC Systems Biology, 12(1), Article 102. https://doi.org/10.1186/s12918-018-0632-4

Baert, J., Delepierre, A., Telek, S., Fickers, P., Toye, D., Delamotte, A., Lara, A. R., Jaén, K. E., Gosset, G., Jensen, P. R. & Delvigne, F. (2016). Microbial population heterogeneity versus bioreactor heterogeneity: Evaluation of Redox Sensor Green as an exogenous metabolic biosensor. Engineering in Life Sciences, 16(7), 643-651. https://doi.org/10.1002/elsc.201500149

Velazquez, D., Jaén, K. E., Sigala, J. C. & Lara, A. R. (2021). Minimized backbone and novel microaerobic promoters boost plasmid DNA production. Process Biochemistry, 106, 130-136. https://doi.org/10.1016/j.procbio.2021.04.013

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Revised 16.01.2025