Publications

All publications

Wunderlich, M., Taymaz-Nikerel, H., Gosset, G., Ramírez, O. T. & Lara, A. R. (2014). Effect of growth rate on plasmid DNA production and metabolic performance ofengineered Escherichia coli strains. Journal of Bioscience and Bioengineering, 117(3), 336-342. https://doi.org/10.1016/j.jbiosc.2013.08.007

Velazquez, D., Sigala, J. C., Martínez, L. M., Gaytán, P., Gosset, G. & Lara, A. R. (2022). Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains. Microbial Cell Factories, 21(1), Article 183. https://doi.org/10.1186/s12934-022-01906-1

Velazquez, D., Jaén, K. E., Sigala, J. C. & Lara, A. R. (2021). Minimized backbone and novel microaerobic promoters boost plasmid DNA production. Process Biochemistry, 106, 130-136. https://doi.org/10.1016/j.procbio.2021.04.013

Taymaz-Nikerel, H. & Lara, A. R. (2022). Vitreoscilla haemoglobin: A tool to reduce overflow metabolism. Microorganisms, 10(1), Article 43. https://doi.org/10.3390/microorganisms10010043

Taymaz-Nikerel, H. & Lara, A. R. (2016). Editorial: Quantitative systems biology for engineering organisms and pathways. Frontiers in Bioengineering and Biotechnology, 4(MAR), Article 22. https://doi.org/10.3389/fbioe.2016.00022

Soto, R., Caspeta, L., Barrón, B., Gosset, G., Ramírez, O. T. & Lara, A. R. (2011). High cell-density cultivation in batch mode for plasmid DNA production by a metabolically engineered E. coli strain with minimized overflow metabolism. Biochemical Engineering Journal, 56(3), 165-171. https://doi.org/10.1016/j.bej.2011.06.003

Sigala, J. C., Quiroz, L., Arteaga, E., Olivares, R., Lara, A. R. & Martinez, A. (2019). Physiological and transcriptional comparison of acetate catabolism between Acinetobacter schindleri ACE and Escherichia coli JM101. FEMS Microbiology Letters, 366(12), Article fnz151. https://doi.org/10.1093/femsle/fnz151

Sigala, J. C., Suárez, B. P., Lara, A. R., Borgne, S. L., Bustos, P., Santamaría, R. I., González, V. & Martinez, A. (2017). Genomic and physiological characterization of a laboratory-isolated Acinetobacter schindleri ACE strain that quickly and efficiently catabolizes acetate. Microbiology (United Kingdom), 163(7), 1052-1064. https://doi.org/10.1099/mic.0.000488

Ramírez, E. A., Velázquez, D. & Lara, A. R. (2016). Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli. Biotechnology Letters , 38(4), 651-657. https://doi.org/10.1007/s10529-015-2017-8

Pohlen, L., García, E., Martínez, L. M., Flores, N., Büchs, J., Gosset, G. & Lara, A. R. (2025). Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression. Biotechnology and Bioengineering, 122(6), 1598-1607. https://doi.org/10.1002/bit.28958

Pablos, T. E., Mora, E. M., Le Borgne, S., Ramírez, O. T., Gosset, G. & Lara, A. R. (2011). Vitreoscilla hemoglobin expression in engineered Escherichia coli: Improved performance in high cell-density batch cultivations. Biotechnology Journal, 6(8), 993-1002. https://doi.org/10.1002/biot.201000405

Pablos, T. E., Olivares, R., Sigala, J. C., Ramírez, O. T. & Lara, A. R. (2016). Toward efficient microaerobic processes using engineered Escherichia coli W3110 strains. Engineering in Life Sciences, 16(7), 588-597. https://doi.org/10.1002/elsc.201500129

Pablos, T. E., Soto, R., Mora, E. M., Le Borgne, S., Ramírez, O. T., Gosset, G. & Lara, A. R. (2012). Enhanced production of plasmid DNA by engineered Escherichia coli strains. Journal of Biotechnology, 158(4), 211-214. https://doi.org/10.1016/j.jbiotec.2011.04.015

Pablos, T. E., Sigala, J. C., Le Borgne, S. & Lara, A. R. (2014). Aerobic expression of Vitreoscilla hemoglobin efficiently reduces overflow metabolism in Escherichia coli. Biotechnology Journal, 9(6), 791-799. https://doi.org/10.1002/biot.201300388

Oliveira, P. H., Mairhofer, J., Alves, P. M., Lara, A. R. & Kontoravdi, C. (2015). Advances in the development of biotherapeutics. BioMed Research International, 2015, Article 793876. https://doi.org/10.1155/2015/793876

Martínez, J. A., Rodriguez, A., Moreno, F., Flores, N., Lara, A. R., Ramírez, O. T., Gosset, G. & Bolivar, F. (2018). Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production. BMC Systems Biology, 12(1), Article 102. https://doi.org/10.1186/s12918-018-0632-4

Mairhofer, J. & Lara, A. R. (2014). Advances in host and vector development for the production of plasmid DNA vaccines. Methods in Molecular Biology, 1139, 505-541. https://doi.org/10.1007/978-1-4939-0345-0_38

Licona-Cassani, C., Lara, A. R., Cabrera-Valladares, N., Escalante, A., Hernández-Chávez, G., Martinez, A., Bolívar, F. & Gosset, G. (2014). Inactivation of pyruvate kinase or the phosphoenolpyruvate: Sugar phosphotransferase system increases shikimic and dehydroshikimic acid yields from glucose in bacillus subtilis. Journal of Molecular Microbiology and Biotechnology, 24(1), 37-45. https://doi.org/10.1159/000355264

Lara, A. R., Utrilla, J., Martínez, L. M., Krausch, N., Kaspersetz, L., Hidalgo, D., Cruz-Bournazou, N., Neubauer, P., Sigala, J. C., Gosset, G. & Büchs, J. (2024). Recombinant protein expression in proteome-reduced cells under aerobic and oxygen-limited regimes. Biotechnology and Bioengineering, 121(4), 1216-1230. https://doi.org/10.1002/bit.28645

Lara, A. R., Kunert, F., Vandenbroucke, V., Taymaz-Nikerel, H., Martínez, L. M., Sigala, J. C., Delvigne, F., Gosset, G. & Büchs, J. (2024). Transport-controlled growth decoupling for self-induced protein expression with a glycerol-repressible genetic circuit. Biotechnology and Bioengineering, 121(6), 1789-1802. https://doi.org/10.1002/bit.28697

Lara, A. R., Galindo, J., Jaén, K. E., Juárez, M. & Sigala, J. C. (2020). Physiological response of escherichia coli W3110 and BL21 to the aerobic expression of vitreoscilla hemoglobin. Journal of Microbiology and Biotechnology, 30(10), 1592-1596. https://doi.org/10.4014/JMB.2004.04030

Lara, A. R. (2011). Recombinant protein production in escherichia coli. Revista Mexicana de Ingeniera Quimica, 10(2), 209-223.

Lara, A. R., Taymaz-Nikerel, H., Mashego, M. R., Van Gulik, W. M., Heijnen, J. J., Ramírez, O. T. & Van Winden, W. A. (2009). Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses. Biotechnology and Bioengineering, 104(6), 1153-1161. https://doi.org/10.1002/bit.22503

Lara, A. R., Vazquez-Limón, C., Gosset, G., Bolívar, F., López-Munguía, A. & Ramírez, O. T. (2006). Engineering Escherichia coli to improve culture performance and reduce formation of by-products during recombinant protein production under transient intermittent anaerobic conditions. Biotechnology and Bioengineering, 94(6), 1164-1175. https://doi.org/10.1002/bit.20954

Lara, A. R., Jaén, K. E., Folarin, O., Keshavarz-Moore, E. & Büchs, J. (2019). Effect of the oxygen transfer rate on oxygen-limited production of plasmid DNA by Escherichia coli. Biochemical Engineering Journal, 150, Article 107303. https://doi.org/10.1016/j.bej.2019.107303

Lara, A. R., Jaén, K. E., Sigala, J. C., Mühlmann, M., Regestein, L. & Büchs, J. (2017). Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli. ACS Synthetic Biology, 6(2), 344-356. https://doi.org/10.1021/acssynbio.6b00233

Lara, A. R., Galindo, E., Ramírez, O. T. & Palomares, L. A. (2006). Living with heterogeneities in bioreactors: Understanding the effects of environmental gradients on cells. Molecular Biotechnology, 34(3), 355-381. https://doi.org/10.1385/MB:34:3:355

Lara, A. R., Velázquez, D., Penella, I., Islas, F., González-De la Rosa, C. H. & Sigala, J. C. (2019). Design of a synthetic miniR1 plasmid and its production by engineered Escherichia coli. Bioprocess and Biosystems Engineering, 42(8), 1391-1397. https://doi.org/10.1007/s00449-019-02129-2

Lara, A. R. & Ramírez, O. T. (2012). Plasmid DNA production for therapeutic applications. In A. Lorence (Ed.), Recombinant Gene Expression: Reviews and Protocols, Third Edition (pp. 271-303) https://doi.org/10.1007/978-1-61779-433-9_14

Lara, A. R., Palomares, L. A. & Ramírez, O. T. (2016). Scale-down: Simulating large-scale cultures in the laboratory. In Industrial Biotechnology: Products and Processes (pp. 55-79). Wiley-VCH Verlag GmbH. https://doi.org/10.1002/9783527807833

Lara, A. R., Leal, L., Flores, N., Gosset, G., Bolívar, F. & Ramírez, O. T. (2006). Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system. Biotechnology and Bioengineering, 93(2), 372-385. https://doi.org/10.1002/bit.20704

Lara, A. R., Knabben, I., Regestein, L., Sassi, J., Caspeta, L., Ramírez, O. T. & Büchs, J. (2011). Comparison of oxygen enriched air vs. pressure cultivations to increase oxygen transfer and to scale-up plasmid DNA production fermentations. Engineering in Life Sciences, 11(4), 382-386. https://doi.org/10.1002/elsc.201000104

Lara, A. R., Jaén, K. E., Sigala, J. C., Regestein, L. & Büchs, J. (2017). Evaluation of microbial globin promoters for oxygen-limited processes using Escherichia coli. Journal of Biological Engineering, 11(1), Article 39. https://doi.org/10.1186/s13036-017-0082-3

Lara, A. R., Caspeta, L., Gosset, G., Bolívar, F. & Ramírez, O. T. (2008). Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: An alternative to fed-batch cultures. Biotechnology and Bioengineering, 99(4), 893-901. https://doi.org/10.1002/bit.21664

Lara, A. R. & Gosset, G. (2019). Minimal cells: Design, construction, biotechnological applications. Springer International Publishing. https://doi.org/10.1007/978-3-030-31897-0

Lara, A. R. & Gosset, G. (2019). Preface. In Minimal Cells: Design, Construction, Biotechnological Applications (pp. v-vi). Springer International Publishing.

Lara, A. R., Andersen, M. B., Madsen, A. A. V., Fønss, K. G., Irla, M., Taymaz-Nikerel, H., Martínez, L. M., Dicke, M. D., Mann, M., Magnus, J. B. & Gosset, G. (2025). Biomanufacturing Potential of Streamlined Cells. Biotechnology and Bioengineering, 122(12), 3309-3318. https://doi.org/10.1002/bit.70070

Krausch, N., Kaspersetz, L., Gaytán-Castro, R. D., Schermeyer, M. T., Lara, A. R., Gosset, G., Cruz Bournazou, M. N. & Neubauer, P. (2023). Model-Based Characterization of E. coli Strains with Impaired Glucose Uptake. Bioengineering, 10(7), Article 808. https://doi.org/10.3390/bioengineering10070808

Knabben, I., Regestein, L., Marquering, F., Steinbusch, S., Lara, A. R. & Büchs, J. (2010). High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. Journal of Biotechnology, 150(1), 73-79. https://doi.org/10.1016/j.jbiotec.2010.07.006

Juárez, M., González-De La Rosa, C. H., Sigala, J. C. & Lara, A. R. (2021). Effect of vitreoscilla hemoglobin on recombinant protein expression and energy and redox state of cho cells. Revista Mexicana de Ingeniera Quimica, 20(1), 281-288. https://doi.org/10.24275/rmiq/Bio1866

Juárez, M., González-De la Rosa, C. H., Memún, E., Sigala, J. C. & Lara, A. R. (2017). Aerobic expression of Vitreoscilla hemoglobin improves the growth performance of CHO-K1 cells. Biotechnology Journal, 12(3), Article 1600438. https://doi.org/10.1002/biot.201600438

Jaén, K. E., Velázquez, D., Sigala, J. C. & Lara, A. R. (2019). Design of a microaerobically inducible replicon for high-yield plasmid DNA production. Biotechnology and Bioengineering, 116(10), 2514-2525. https://doi.org/10.1002/bit.27091

Jaén, K. E., Sigala, J. C., Olivares-Hernández, R., Niehaus, K. & Lara, A. R. (2017). Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli. BMC Biotechnology, 17(1), Article 60. https://doi.org/10.1186/s12896-017-0378-x

Jaén, K. E., Velazquez, D., Delvigne, F., Sigala, J. C. & Lara, A. R. (2019). Engineering E. coli for improved microaerobic pDNA production. Bioprocess and Biosystems Engineering, 42(9), 1457-1466. https://doi.org/10.1007/s00449-019-02142-5

Jaén, K. E., Lara, A. R. & Ramírez, O. T. (2013). Effect of heating rate on pDNA production by E. coli. Biochemical Engineering Journal, 79, 230-238. https://doi.org/10.1016/j.bej.2013.08.006

Jaén, K. E., Velazquez, D., Sigala, J. C. & Lara, A. R. (2021). Enhancing microaerobic plasmid DNA production by chromosomal expression of Vitreoscilla hemoglobin in E. coli. Biochemical Engineering Journal, 166, Article 107862. https://doi.org/10.1016/j.bej.2020.107862

Islas, F., Sabido, A., Sigala, J. C. & Lara, A. R. (2023). Design of microaerobically inducible miniR1 plasmids. mLife, 2(1), 101-104. https://doi.org/10.1002/mlf2.12058

Grijalva-Hernández, F., Vega-Estrada, J., Escobar-Rosales, M., Ortega-López, J., Aguilar-López, R., Lara, A. R. & Montes-Horcasitas, M. D. C. (2019). High kanamycin concentration as another stress factor additional to temperature to increase pdna production in e. Coli dh5α batch and fed-batch cultures. Microorganisms, 7(12), Article 711. https://doi.org/10.3390/microorganisms7120711

Gálvez, R. M., Pablos, T. E., Sigala, J. C. & Lara, A. R. (2014). Co-utilization of glucose and xylose increases growth rate without affecting plasmid dna yield of engineered E. coli. Revista Mexicana de Ingeniera Quimica, 13(2), 387-391.

Galindo, J., Barrón, B. L. & Lara, A. R. (2016). Improved production of large plasmid DNA by enzyme-controlled glucose release. Annals of Microbiology, 66(3), 1337-1342. https://doi.org/10.1007/s13213-016-1218-2

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Revised 16.01.2025